Overview

  • Product name
    Anti-Acetylcholinesterase antibody [ZR3]
    See all Acetylcholinesterase primary antibodies
  • Description
    Mouse monoclonal [ZR3] to Acetylcholinesterase
  • Specificity
    This antibody reacts with both the soluble and membrane-associated AChE.
  • Tested applications
    Suitable for: Inhibition Assay, IHC-Fr, IP, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat, Rabbit, Guinea pig, Cat, Human
  • Immunogen

    Other Immunogen Type corresponding to Rat Acetylcholinesterase. Purified rat brain acetylcholinesterase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    ZR3
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Immunohistochemical staining of Ach in rat brain results in staining of nerve fibers and terminals.

IP 1/1000.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
    • Tissue specificity
      Isoform H is highly expressed in erythrocytes.
    • Sequence similarities
      Belongs to the type-B carboxylesterase/lipase family.
    • Cellular localization
      Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACEE antibody
      • ACES_HUMAN antibody
      • Acetylcholinesterase antibody
      • AChE antibody
      • Apoptosis related acetylcholinesterase antibody
      • ARACHE antibody
      • N ACHE antibody
      • N-ACHE antibody
      • YT antibody
      • YT blood group antibody
      see all

    Images

    • Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in C6 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) ab2802 shows staining in U251 glioma cells. Acetylcholinesterase staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase ab2802 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    References

    This product has been referenced in:
    • Motamed-Khorasani A  et al. Differentially androgen-modulated genes in ovarian epithelial cells from BRCA mutation carriers and control patients predict ovarian cancer survival and disease progression. Oncogene 26:198-214 (2007). Read more (PubMed: 16832351) »
    • Rakonczay Z & Brimijoin S Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species. J Neurochem 46:280-7 (1986). Read more (PubMed: 3510009) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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