Overview

  • Product nameAnti-ACF1 / BAZ1A antibody
    See all ACF1 / BAZ1A primary antibodies
  • Description
    Mouse polyclonal to ACF1 / BAZ1A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein:

    PTALPPSEPKVDQKLPRSSERRGSGGGTQFPARSRAVAAGEAAARGAAGP ERGSPLGRRVSPRCLCSGEGGQVAVGVIAGKRGRRGRDGSRRAPGGREMP

    , corresponding to amino acids 53/152 of Human BAZ1A

  • General notesProduced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferConstituents: 50% Glycerol
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 178 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionComponent of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
    Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
  • Tissue specificityHighly expressed in testis and at low or undetectable levels in other tissues analyzed.
  • Sequence similaritiesBelongs to the WAL family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 WAC domain.
  • Cellular localizationNucleus. May target the CHRAC complex to heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acf1 antibody
    • ACF1, drosophila, homolog of antibody
    • ATP dependent chromatin remodelling protein antibody
    • ATP utilizing chromatin assembly and remodeling factor 1 antibody
    • ATP-dependent chromatin-remodeling protein antibody
    • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
    • B930060C03 antibody
    • Baz1a antibody
    • BAZ1A_HUMAN antibody
    • BC065123 antibody
    • Bromodomain adjacent to zinc finger domain 1A antibody
    • Bromodomain adjacent to zinc finger domain protein 1A antibody
    • cbp146 antibody
    • CHRAC subunit ACF1 antibody
    • DKFZp586E0518 antibody
    • FLJ14383 antibody
    • Gtl5 antibody
    • hACF1 antibody
    • hWALp1 antibody
    • WALp1 antibody
    • WCRF180 antibody
    • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
    • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
    see all

Anti-ACF1 / BAZ1A antibody images

  • All lanes : Anti-ACF1 / BAZ1A antibody (ab22233) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 178 kDa

References for Anti-ACF1 / BAZ1A antibody (ab22233)

ab22233 has not yet been referenced specifically in any publications.

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