The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression. Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
Tissue specificityHighly expressed in testis and at low or undetectable levels in other tissues analyzed.
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Anti-ACF1 / BAZ1A antibody images
Western blot - Anti-ACF1 / BAZ1A antibody (ab56843)
Lane 1: Wild-type HAP1 cell lysate (40 µg) Lane 2: BAZ1A knockout HAP1 cell lysate (40 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab56843 observed at 170 kDa. Red - loading control, ab176560, observed at 52 kDa.
ab56843 was shown to recognize ACF1 / BAZ1A when ACF1 / BAZ1A knockout samples were used, along with additional cross-reactive bands. Wild-type and ACF1 / BAZ1A knockout samples were subjected to SDS-PAGE. Ab56843 and ab176560 (loading control to GAPDH) were diluted at 1 µg/ml and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
Western blot - ACF1 / BAZ1A antibody (ab56843)
Western blot against tagged recombinant protein immunogen using ab56843 ACF1 / BAZ1A antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-ACF1 / BAZ1A antibody (ab56843)
has not yet been referenced specifically in any publications.
Publishing research using ab56843? Please let us know so that we can cite the reference in this datasheet.
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