• Product name
    Anti-ACF1 / BAZ1A antibody
    See all ACF1 / BAZ1A primary antibodies
  • Description
    Rabbit polyclonal to ACF1 / BAZ1A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 1260-1309 (LPKRGRPQVR LPVKTRGKLS SSFSSRGQQQ EPGRYPSRSQ QSTPKTTVSS) of Human ACF1/ BAZ1A (NP_038476).

  • Positive control
    • Human fetal stomach lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab94749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 179 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
    Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
  • Tissue specificity
    Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
  • Sequence similarities
    Belongs to the WAL family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 WAC domain.
  • Cellular localization
    Nucleus. May target the CHRAC complex to heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acf1 antibody
    • ACF1, drosophila, homolog of antibody
    • ATP dependent chromatin remodelling protein antibody
    • ATP utilizing chromatin assembly and remodeling factor 1 antibody
    • ATP-dependent chromatin-remodeling protein antibody
    • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
    • B930060C03 antibody
    • Baz1a antibody
    • BAZ1A_HUMAN antibody
    • BC065123 antibody
    • Bromodomain adjacent to zinc finger domain 1A antibody
    • Bromodomain adjacent to zinc finger domain protein 1A antibody
    • cbp146 antibody
    • CHRAC subunit ACF1 antibody
    • DKFZp586E0518 antibody
    • FLJ14383 antibody
    • Gtl5 antibody
    • hACF1 antibody
    • hWALp1 antibody
    • WALp1 antibody
    • WCRF180 antibody
    • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
    • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
    see all

Anti-ACF1 / BAZ1A antibody images

  • Anti-ACF1 / BAZ1A antibody (ab94749) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 179 kDa

References for Anti-ACF1 / BAZ1A antibody (ab94749)

This product has been referenced in:
  • Klement K  et al. Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair. J Cell Biol 207:717-33 (2014). Read more (PubMed: 25533843) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I am sorry this antibody is not working in WB. Could you please provide a few additional details about your protocol and results?

1) When you say that the antibody failed in WB, were you obtaining high background,...

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