Overview

  • Product nameAnti-Acid Phosphatase 2 antibody
    See all Acid Phosphatase 2 primary antibodies
  • Description
    Mouse monoclonal to Acid Phosphatase 2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human, Chimpanzee, Ferret, Chlamydia psittaci, Hepatitis B virus, Orangutan, Scorpion
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-424 of Human Acid Phosphatase 2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typelambda
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Involvement in diseaseDefects in ACP2 are a cause of acid phosphatase deficiency (ACPHD) [MIM:200950]. The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues.
    • Sequence similaritiesBelongs to the histidine acid phosphatase family.
    • Post-translational
      modifications
      The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.
      N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used.
    • Cellular localizationLysosome membrane. Lysosome lumen. The soluble form arises by proteolytic processing of the membrane-bound form.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid phosphatase 2 lysosomal antibody
      • ACP 2 antibody
      • ACP2 antibody
      • LAP antibody
      • Lysosomal acid phosphatase 2 antibody
      • Lysosomal acid phosphatase antibody
      • PPAL_HUMAN antibody
      see all

    Anti-Acid Phosphatase 2 antibody images

    • Acid Phosphatase 2 antibody (ab54721) used in immunofluorescence at 10ug/ml on HeLa cells.
    • Acid Phosphatase 2 antibody (ab54721) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.
    • Western blot against tagged recombinant protein immunogen using ab54721 Acid Phosphatase 2 antibody at 1ug/ml. Predicted band size of immunogen is 72 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • ab54721 at 10 ug/ml staining Acid Phosphatase 2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    References for Anti-Acid Phosphatase 2 antibody (ab54721)

    ab54721 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab54721.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"