Overview

  • Product nameAnti-Acid Phosphatase 2 antibody
    See all Acid Phosphatase 2 primary antibodies
  • Description
    Rabbit polyclonal to Acid Phosphatase 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (VPITEDRLLK FPLGPCPRYE QLQNETRQTP EYQNESSRNA QFLDMVANET) of Human Acid Phosphatase 2 (NP_001601).

  • Positive control
    • Human Fetal Brain Lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Involvement in diseaseDefects in ACP2 are a cause of acid phosphatase deficiency (ACPHD) [MIM:200950]. The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues.
  • Sequence similaritiesBelongs to the histidine acid phosphatase family.
  • Post-translational
    modifications
    The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.
    N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used.
  • Cellular localizationLysosome membrane. Lysosome lumen. The soluble form arises by proteolytic processing of the membrane-bound form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid phosphatase 2 lysosomal antibody
    • ACP 2 antibody
    • ACP2 antibody
    • LAP antibody
    • Lysosomal acid phosphatase 2 antibody
    • Lysosomal acid phosphatase antibody
    • PPAL_HUMAN antibody
    see all

Anti-Acid Phosphatase 2 antibody images

  • Anti-Acid Phosphatase 2 antibody (ab84896) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human Fetal Brain Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Acid Phosphatase 2 with ab84896 at 4-8µg/ml.

References for Anti-Acid Phosphatase 2 antibody (ab84896)

ab84896 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"