Overview

  • Product name
    Anti-Acid Phosphatase antibody
    See all Acid Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Acid Phosphatase
  • Specificity
    This antibody reacts with erythrocyte acid phosphatase.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Pig
  • Immunogen

    A region within synthetic peptide: SENWRVDSAA TSGYEIGNPP DYRGQSCMKR HGIPMSHVAR QITKEDFATF, corresponding to N terminal amino acids 37-86 of Human Acid Phosphatase

  • Positive control
    • Jurkat cell lysate, human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab58688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • Tissue specificity
    T-lymphocytes express only isoform 2.
  • Sequence similarities
    Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid phosphatase 1 soluble antibody
    • Acid phosphatase of erythrocyte antibody
    • ACP1 antibody
    • Adipocyte acid phosphatase antibody
    • Cytoplasmic phosphotyrosyl protein phosphatase antibody
    • HAAP antibody
    • LMW-PTP antibody
    • LMW-PTPase antibody
    • Low molecular weight cytosolic acid phosphatase antibody
    • Low molecular weight phosphotyrosine protein phosphatase antibody
    • PAP1 antibody
    • PAP2 antibody
    • phosphatase, acid, of erythrocyte antibody
    • PPAC_HUMAN antibody
    • Protein tyrosine phosphatase antibody
    • PTPase antibody
    • Purple acid phosphatase antibody
    • Red cell acid phosphatase 1 antibody
    • testicular secretory protein Li 37 antibody
    see all

Images

  • Anti-Acid Phosphatase antibody (ab58688) at 1.25 µg/ml + Jurkat cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 dilution.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Acid Phosphatase with ab58688 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab58688 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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