Overview

  • Product nameAnti-Acid Phosphatase antibody
    See all Acid Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Acid Phosphatase
  • SpecificityThis antibody reacts with erythrocyte acid phosphatase.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Pig
  • Immunogen

    A region within synthetic peptide: SENWRVDSAA TSGYEIGNPP DYRGQSCMKR HGIPMSHVAR QITKEDFATF, corresponding to N terminal amino acids 37-86 of Human Acid Phosphatase

  • Positive control
    • Jurkat cell lysate, human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-Acid Phosphatase antibody images

  • Anti-Acid Phosphatase antibody (ab58688) at 1.25 µg/ml + Jurkat cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 dilution.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Acid Phosphatase with ab58688 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-Acid Phosphatase antibody (ab58688)

ab58688 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"