Abcam's Acid Phosphatase Assay Kit (Colorimetric) is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP.
Visit our FAQs page for tips and troubleshooting.
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.
|AP Assay buffer||NM||1 x 100ml|
|AP Enzyme (Lyophilised)||Green||1 vial|
|pNPP Substrate (10 tablets)||Red||1 vial|
|Stop Solution||WM||1 x 10ml|
Our Abpromise guarantee covers the use of ab83367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|