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Abcam's Acid Phosphatase Assay Kit (Colorimetric) is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP.
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Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.
|AP Assay buffer||NM||1 x 100ml|
|AP Enzyme (Lyophilised)||Green||1 vial|
|pNPP Substrate (10 tablets)||Red||1 vial|
|Stop Solution||WM||1 x 10ml|
Our Abpromise guarantee covers the use of ab83367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|