Overview

  • Product nameAcid Phosphatase Assay Kit (Colorimetric)See all Acid phosphatase kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.001 mM
  • Range
    0.001 mM - 1 mM
  • Product overview

    Abcam's Acid Phosphatase Assay Kit (Colorimetric) is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceAcid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
  • Cellular localizationACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
  • Alternative names
    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

Applications

Our Abpromise guarantee covers the use of ab83367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Acid Phosphatase Assay Kit (Colorimetric) images

  • p-Nitrophenol (pNP) standard curve
  • AP Enzyme samples 40 minute assay

Protocols

References for Acid Phosphatase Assay Kit (Colorimetric) (ab83367)

This product has been referenced in:
  • Rhee I  et al. Macrophage fusion is controlled by the cytoplasmic protein tyrosine phosphatase PTP-PEST/PTPN12. Mol Cell Biol 33:2458-69 (2013). Functional Studies . Read more (PubMed: 23589331) »

See 1 Publication for this product

Product Wall



If the TRAP is able to perform the same functions (phosphorylation of the substrates) as any other AP, then yes, this kit could be used. However, we cannot guarantee that it will definitely work as we have never tried it to quantify TRAP.
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Thank you for your enquiry.

Unfortunately, most of the information you asked for is proprietary info. However, I can confirm that the assay buffer does not contain protease inhibitors and its pH is 5.0.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"