Acid Phosphatase Assay Kit (Fluorometric) (ab83370)

Overview

  • Product name
    Acid Phosphatase Assay Kit (Fluorometric)
    See all Acid phosphatase kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    0.001 mU/ml
  • Product overview

    Abcam's Acid Phosphatase Assay Kit (Fluorometric) uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.

    The kit is suitable for both research and drug discovery.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 500 tests
    AP Assay buffer NM 1 x 100ml
    AP Enzyme (Lyophilised) Green 1 vial
    MUP Substrate Red 1 vial
    Stop Solution WM 1 x 25ml
  • Research areas
  • Relevance
    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
  • Cellular localization
    ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
  • Alternative names
    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

Applications

Our Abpromise guarantee covers the use of ab83370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • 4-Methylumbelliferone (4-MU) Standard Curve.

Protocols

References

ab83370 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


For Acid Phosphatase Assay Kit ab83370, the pH of the buffer prevents alkaline phosphatase from working (ALP can only function in an alkaline pH whereas AP works in an acidic pH) so you only measure AP activity.
Acid phosphatase (AP) can depho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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