The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificityExpressed in both non-smooth muscle tissues as well as smooth muscle tissues.
Sequence similaritiesBelongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.