Overview

  • Product name
    Anti-Acidic Calponin antibody
    See all Acidic Calponin primary antibodies
  • Description
    Rabbit polyclonal to Acidic Calponin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 68-312 of Human Acidic Calponin (Uniprot ID: Q15417).

  • Positive control
    • 293T, HeLa, HepG2, NIH 3T3 and JC whole cell lysates; Human U87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-Acidic Calponin antibody (ab151427) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
  • All lanes : Anti-Acidic Calponin antibody (ab151427) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
  • Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling Acidic Calponin with ab151427 at 1/500 dilution.

References

ab151427 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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