Overview

  • Product nameAnti-Acidic Cytokeratin antibody [AE1]
    See all Acidic Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE1] to Acidic Cytokeratin
  • Specificityab80824 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of the acidic subfamily.
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Monkey
  • Immunogen

    Human epidermal keratin.

  • Positive control
    • Skin. Squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Acidic Cytokeratin antibody [AE1] images

    • Formalin-fixed, paraffin-embedded human squamous cell carcinoma of lung stained with ab80824 at 1/50 dilution using a peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

    References for Anti-Acidic Cytokeratin antibody [AE1] (ab80824)

    This product has been referenced in:
    • Cooper D  et al. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation 28:30-5 (1984). Read more (PubMed: 6083891) »
    • Eichner R  et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Read more (PubMed: 6201491) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab80824.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"