The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.5 - 1 µg/ml. Detects Acinus L, Acinus S and Acinus S' at 220, 98 and 94 kDa respectively, in RIPA lysates from Jurkat cells. Detection of Acinus L was lost in Jurkat cells treated with 0.5 µM staurosporin for 6 hours. Loss of Acinus L is observed upon induction of apoptosis.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
Sequence similaritiesContains 1 SAP domain.
Post-translational modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.