Overview

  • Product name
  • Description
    Rabbit polyclonal to Acinus
  • Specificity
    The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DTSENRPENDVPEPP

    , corresponding to amino acids 775-789 of Human Acinus L, 48 to 62 of human Acinus S’, or 17 to 31 of human Acinus S, which differ from those of mouse Acinus by one amino acid.

  • Positive control
    • K562 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab7354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Contains 1 SAP domain.
    • Post-translational
      modifications
      Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACIN 1 antibody
      • ACIN1 antibody
      • ACINU_HUMAN antibody
      • Acinus antibody
      • ACN antibody
      • Apoptotic chromatin condensation inducer 1 antibody
      • Apoptotic chromatin condensation inducer in the nucleus antibody
      • DKFZp667N107 antibody
      • fSAP152 antibody
      • functional spliceosome-associated protein 152 antibody
      • KIAA0670 antibody
      see all

    Images

    • Immunofluorescence of Acinus in K562 cells with ab7354 at 20 ug/mL.

    • Anti-Acinus antibody (ab7354) at 1 µg/ml + K562 whole cell lysate

      Predicted band size : 151 kDa
    • ab7354 at 5µg/ml staining Acinus in K562 cells by ICC/IF

    References

    ab7354 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab7354.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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