Overview

  • Product nameAnti-ACK1 (phospho Y284) antibody
    See all ACK1 primary antibodies
  • Description
    Rabbit polyclonal to ACK1 (phospho Y284)
  • Specificityab74091 detects ACK1 only when phosphorylated at tyrosine 284.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).

  • Positive control
    • Human breast carcinoma tissue, A549 cells, and HepG2 cell extracts treated with EGF (200ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab74091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately >117 kDa (predicted molecular weight: 114 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionNon-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
  • Tissue specificityThe Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • DomainThe EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
    The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
    The UBA domain binds both poly- and mono-ubiquitin.
  • Post-translational
    modifications
    Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
    Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes.
  • Cellular localizationCell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetate kinase 1 antibody
    • Acetokinase 1 antibody
    • ACK 1 antibody
    • ACK antibody
    • ACK-1 antibody
    • ACK1 antibody
    • ACK1_HUMAN antibody
    • Activated Cdc42 associated kinase 1 antibody
    • Activated CDC42 kinase 1 antibody
    • Activated p21cdc42Hs kinase antibody
    • FLJ44758 antibody
    • FLJ45547 antibody
    • p21cdc42Hs antibody
    • TNK 2 antibody
    • TNK2 antibody
    • Tyrosine kinase non receptor 2 antibody
    • Tyrosine kinase non receptor protein 2 antibody
    • Tyrosine kinase non-receptor protein 2 antibody
    see all

Anti-ACK1 (phospho Y284) antibody images

  • ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab74091 at 1/50 dilution staining ACK1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-ACK1 (phospho Y284) antibody (ab74091) at 1/500 dilution

    Lane 1 : HepG2 cell extracts treated with EGF (200ng/ml, 30mins)
    Lane 2 : HepG2 cell extracts treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : >117 kDa (why is the actual band size different from the predicted?)

References for Anti-ACK1 (phospho Y284) antibody (ab74091)

This product has been referenced in:

See 1 Publication for this product

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