Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
Key features and details
- Mouse monoclonal [6D1BE4] to Aconitase 2
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Aconitase 2 antibody [6D1BE4]
See all Aconitase 2 primary antibodies -
Description
Mouse monoclonal [6D1BE4] to Aconitase 2 -
Host species
Mouse -
Specificity
This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting. -
Tested applications
Suitable for: IP, ICC/IF, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purification notes
ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
6D1BE4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
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ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: the target protein locates to the mitochondria. -
Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320. -
ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110320 has not yet been referenced specifically in any publications.