Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

Overview

  • Product nameAnti-Aconitase 2 antibody [6D1BE4]
    See all Aconitase 2 primary antibodies
  • Description
    Mouse monoclonal [6D1BE4] to Aconitase 2
  • SpecificityThis antibody is not suitable for Western blot. We recommend ab110321 for Western blotting.
  • Tested applicationsSuitable for: IP, ICC/IF, In-Cell ELISA, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Borvine heart mitochondria

  • Positive control
    • HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for WB.
  • Target

    Anti-Aconitase 2 antibody [6D1BE4] images

    • ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
      Note: the target protein locates to the mitochondria.
    • Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
      Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320.

    • ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

    References for Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

    ab110320 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"