Overview

  • Product name
  • Description
    Rabbit polyclonal to Aconitase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) from the N-terminus conjugated to KLH

  • Positive control
    • Rat heart whole tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Target

  • Function
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
  • Sequence similarities
    Belongs to the aconitase/IPM isomerase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACO 2 antibody
    • ACO2 antibody
    • ACON_HUMAN antibody
    • aconitase 2 antibody
    • Aconitase 2 mitochondrial antibody
    • Aconitase antibody
    • Aconitase2 antibody
    • Aconitate hydratase antibody
    • Aconitate hydratase mitochondrial antibody
    • ACONM antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • ICRD antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-Aconitase 2 antibody (ab71439) at 1/1000 dilution

    Lane 1 : Rat heart whole tissue lysate lysed with 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail
    Lane 2 : Rat heart whole tissue lysate lysed with T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

References

This product has been referenced in:
  • Froehlich JM  et al. In vitro indeterminate teleost myogenesis appears to be dependent on Pax3. In Vitro Cell Dev Biol Anim 49:371-85 (2013). Read more (PubMed: 23613306) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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