All lanes : Anti-Aconitase 2 antibody (ab71439) at 1/1000 dilution
Lane 1 : Rat heart whole tissue lysate lysed with 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail Lane 2 : Rat heart whole tissue lysate lysed with T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail
Lysates/proteins at 12.5 µg per lane.
Predicted band size : 85 kDa Observed band size : 85 kDa
References for Anti-Aconitase 2 antibody (ab71439)
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