• Product nameAnti-Aconitase 2 antibody
    See all Aconitase 2 primary antibodies
  • Description
    Rabbit polyclonal to Aconitase 2
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Caenorhabditis elegans
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (


    ) of human ACO2 (NP_001089).

  • Positive control
    • 721_B cell lysate



Our Abpromise guarantee covers the use of ab83528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-Aconitase 2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Aconitase 2 with ab83528 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of the cell bodies of penealocytes and connective tissue (septum). Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Aconitase 2. Right - Merge.
  • Anti-Aconitase 2 antibody (ab83528) at 1 µg/ml (in 5% skim milk/ PBS) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • ICC/IF image of ab83528 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83528, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Aconitase 2 antibody (ab83528)

This product has been referenced in:
  • Fischer F  et al. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep 5:12697 (2015). Read more (PubMed: 26220011) »
  • Scheving R  et al. Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve. J Proteomics 75:3987-4004 (2012). WB, ELISA ; Mouse . Read more (PubMed: 22588120) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Caenorhabditis elegans Cell lysate - whole cell (Whole worm homogenate)
Loading amount 80 µg
Specification Whole worm homogenate
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Michal Bejerano-Sagie

Verified customer

Submitted Jan 17 2012

DISCOUNT CODE: 41541 Expiration date: 17/04/2012 I am very pleased to hear you would like to accept our offer and test ab83528 in C.elegans. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, pleas...

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Thank you for your enquiry and interest. The cross-reactivity of this antibody with C.elegans has not been tested, though it is predicted due to sequence homology. I could certainly offer a discount code for testing. Could you please pass the custo...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole flies)
Loading amount 15 µg
Specification whole flies
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 21 2011