Overview

  • Product name
  • Description
    Rabbit polyclonal to ACOT1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 183-421 of Human ACOT1 (BC132889).

  • Positive control
    • Human Fetal Skin, Liver, Heart and Thymus IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab100915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500.

Target

  • Relevance
    Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • ACH2 antibody
    • Acyl CoA thioesterase 1, cytosolic antibody
    • Acyl coenzyme A thioesterase 1 antibody
    • CTE 1 antibody
    • CTE I antibody
    • CTE Ib antibody
    • Cte1 antibody
    • Cytosolic acyl CoA thioesterase 1 antibody
    • D12Ucla1 antibody
    • Inducible cytosolic acyl-coenzyme A thioester hydrolase antibody
    • Long chain acyl CoA hydrolase antibody
    • Long chain acyl CoA thioester hydrolase antibody
    see all

Images

  • All lanes : Anti-ACOT1 antibody (ab100915) at 1/1000 dilution

    Lane 1 : Human Fetal Skin Lysate
    Lane 2 : Human Fetal Liver Lysate
    Lane 3 : Human Fetal Heart Lysate


    Predicted band size : 46 kDa
  • ab100915, at 1/100 dilution, staining cytoplasmic ACOT1 in formalin-fixed, paraffin-embedded Human fetal thymus tissue, by Immunohistochemistry.
  • ICC/IF image of ab100915 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100915, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Jeckel KM  et al. Dietary fatty acids alter left ventricular myocardial gene expression in Wistar rats. Nutr Res 34:694-706 (2014). WB ; Rat . Read more (PubMed: 25172377) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
30 µg
Treatment
Fed, Fasted, Fasted on HFD
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
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Abcam user community

Verified customer

Submitted Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse intestinal organoids)
Permeabilization
Yes - 0.5%TritonX, 3%FCS in PBS
Specification
Mouse intestinal organoids
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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