Overview

  • Product name
  • Description
    Rabbit polyclonal to ACOT12
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SASRLCWAHP FLKSVDMFKF RGPSTVGDRL VFTAIVNNTF QTCVEVGVRV) of human ACOT12 (NP_570123).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based ELISA is 1:12500.

Target

  • Function
    Hydrolyzes acetyl-CoA to acetate and CoA.
  • Pathway
    Carbohydrate metabolism; pyruvate metabolism.
  • Sequence similarities
    Contains 2 acyl coenzyme A hydrolase domains.
    Contains 1 START domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACO12_HUMAN antibody
    • Acot12 antibody
    • Acyl CoA thioester hydrolase 12 antibody
    • Acyl CoA thioesterase 12 antibody
    • Acyl-CoA thioester hydrolase 12 antibody
    • Acyl-CoA thioesterase 12 antibody
    • Acyl-coenzyme A thioesterase 12 antibody
    • Cach antibody
    • CACH-1 antibody
    • CACH1 antibody
    • Cytoplasmic acetyl CoA hydrolase 1 antibody
    • Cytoplasmic acetyl-CoA hydrolase 1 antibody
    • cytosolic acetyl CoA hydrolase antibody
    • hCACH-1 antibody
    • hCACH1 antibody
    • MGC105114 antibody
    • StAR related lipid transfer (START) domain containing 15 antibody
    • StARD15 antibody
    • START domain containing protein 12 antibody
    • START domain-containing protein 15 antibody
    • THEAL antibody
    see all

Images

  • Anti-ACOT12 antibody (ab83326) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References

ab83326 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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