Overview

  • Product nameAnti-ACOT7 antibody
    See all ACOT7 primary antibodies
  • Description
    Rabbit polyclonal to ACOT7
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide from near the C terminal residues of human ACOT7 (NM_181864).

  • Positive control
    • Human fetal brain lysate; human brain tissue. IF/ICC: Malme-3m cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab85151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/200.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA.
  • Tissue specificityIsoform 4 is expressed exclusively in brain.
  • Sequence similaritiesContains 2 acyl coenzyme A hydrolase domains.
  • Cellular localizationCytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACH1 antibody
    • ACOT 7 antibody
    • ACOT7 antibody
    • ACT antibody
    • Acyl CoA thioesterase 7 antibody
    • Acyl CoA thioesterase long chain antibody
    • Acyl-CoA thioesterase 7 antibody
    • BACH antibody
    • BACH_HUMAN antibody
    • Brain acyl CoA hydrolase antibody
    • Brain acyl-CoA hydrolase antibody
    • CTE-II antibody
    • CTE-IIa antibody
    • Cytosolic acyl coenzyme A thioester hydrolase antibody
    • hBACH antibody
    • LACH antibody
    • LACH1 antibody
    • Long chain acyl-CoA thioester hydrolase antibody
    see all

Anti-ACOT7 antibody images

  • Anti-ACOT7 antibody (ab85151) at 1/500 dilution + human fetal brain lysate

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab85151, at a 1/100 dilution, staining ACOT7 in formalin fixed, paraffin embedded human brain tissue by Immunohistochemistry.
  • ICC/IF image of ab85151 stained Malme-3m cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85151, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ACOT7 antibody (ab85151)

ab85151 has not yet been referenced specifically in any publications.

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