Overview

  • Product name
  • Description
    Goat polyclonal to ACOX2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide:

    C-HQSRLRPSDPEAK

    , corresponding to internal sequence amino acids 325-336 of Human ACOX2 (NP_003491.1).

  • Positive control
    • Human Liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).
    An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids.
    • Tissue specificity
      Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.
    • Sequence similarities
      Belongs to the acyl-CoA oxidase family.
    • Cellular localization
      Peroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase antibody
      • 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase antibody
      • 3 alpha 7 alpha 12 alpha trihydroxy 5 beta cholestanoyl CoA oxidase antibody
      • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholestanoyl CoA 24 hydroxylase antibody
      • 3-alpha antibody
      • 7-alpha antibody
      • ACOX 2 antibody
      • ACOX2 antibody
      • ACOX2_HUMAN antibody
      • Acyl CoA oxidase 2 antibody
      • Acyl Coenzyme A oxidase 2 antibody
      • Acyl Coenzyme A oxidase 2 branched chain antibody
      • Acyl coenzyme A oxidase 2 peroxisomal antibody
      • BCOX antibody
      • BRCACOX antibody
      • BRCOX antibody
      • Peroxisomal acyl-coenzyme A oxidase 2 antibody
      • Peroxisomal branched chain acyl CoA oxidase antibody
      • THCA CoA oxidase antibody
      • THCA-CoA oxidase antibody
      • THCCox antibody
      • Trihydroxycoprostanoyl CoA oxidase antibody
      • Trihydroxycoprostanoyl-CoA oxidase antibody
      see all

    Images

    • Anti-ACOX2 antibody (ab77251) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

      Predicted band size : 77 kDa
      Observed band size : 77 kDa
      Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    References

    ab77251 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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