The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).
An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionOxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids.
Tissue specificityPresent in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.
Sequence similaritiesBelongs to the acyl-CoA oxidase family.