Overview

  • Product nameAnti-ACPT antibody
    See all ACPT primary antibodies
  • Description
    Rabbit polyclonal to ACPT
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 16 amino acid peptide from internal residues of Human ACPT protein.

  • Positive control
    • WB: Human fetal testis, muscle and heart lysates IHC/P: Human fetal testis tissue IF/ICC: U-87 MG cell line.

Properties

  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500.

Target

  • RelevanceAcid phosphatases are enzymes capable of hydrolyzing orthophosphoric acid esters in an acid medium. This gene is upregulated by androgens and is down-regulated by estrogens in the prostate cancer cell line. ACPT dephosphorylates receptor tyrosine-protein kinase erbB-4 and inhibits the ligand-induced proteolytic cleavage. ACPT is predominantly expressed in testis but exhibits a lower level of expression in testicular cancer tissues than in normal tissues. ACPT has structural similarity to prostatic and lysosomal acid phosphatases. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
  • Cellular localizationMembrane; Single pass type I membrane protein
  • Database links
  • Alternative names
    • Acid phosphatase testicular antibody
    • Testicular acid phosphatase antibody

Anti-ACPT antibody images

  • ab100849 stained U-87 MG  cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100849 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-ACPT antibody (ab100849) at 1/500 dilution

    Lane 1 : Human fetal testis lysate
    Lane 2 : Human fetal muscle lysate
    Lane 3 : Human fetal heart lysate


    Predicted band size : 46 kDa
  • ab100849, at a 1/100 dilution, staining ACPT in formalin fixed, paraffin embedded Human fetal testis by Immunohistochemistry.

References for Anti-ACPT antibody (ab100849)

ab100849 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"