Anti-ACRBP antibody (ab64809)
Key features and details
- Rabbit polyclonal to ACRBP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACRBP antibody -
Description
Rabbit polyclonal to ACRBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide derived from an internal region of human ACRBP.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin. -
Tissue specificity
Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers. -
Post-translational
modificationsThe N-terminus is blocked.
Phosphorylated on Tyr residues in capacitated sperm. -
Cellular localization
Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome. Colocalizes with proacrosin in the acrosome of sperm. - Information by UniProt
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Database links
- Entrez Gene: 84519 Human
- Omim: 608352 Human
- SwissProt: Q8NEB7 Human
- Unigene: 123239 Human
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Alternative names
- ACRBP antibody
- ACRBP_HUMAN antibody
- acrosin binding protein antibody
see all
Images
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IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution
Lane 1 : Extracts of 293 cells
Lane 2 : Extracts of HepG2 cells
Lane 3 : Extracts of Jurkat cells
Lane 4 : Extracts of Jurkat cells with immunizing Peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab64809 has been referenced in 4 publications.
- Lin L et al. Cancer-testis antigen ACRBP expression and serum immunoreactivity in ovarian cancer: Its association with prognosis. Immun Inflamm Dis 9:1759-1770 (2021). PubMed: 34528758
- Liu X et al. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics 15:27 (2018). PubMed: 30166971
- Li X et al. Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma. Oncol Lett 13:3080-3086 (2017). PubMed: 28529561
- Cen YH et al. Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells. Cell Biol Int 36:917-22 (2012). PubMed: 22651134