Overview

  • Product name
  • Description
    Rabbit polyclonal to ACRBP
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal region of human ACRBP.

  • Positive control
    • 293, HepG2 and Jurkat cells This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2

Properties

Applications

Our Abpromise guarantee covers the use of ab64809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin.
  • Tissue specificity
    Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.
  • Post-translational
    modifications
    The N-terminus is blocked.
    Phosphorylated on Tyr residues in capacitated sperm.
  • Cellular localization
    Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome. Colocalizes with proacrosin in the acrosome of sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACRBP antibody
    • ACRBP_HUMAN antibody
    • acrosin binding protein antibody
    • Acrosin binding protein precursor antibody
    • Acrosin-binding protein antibody
    • Cancer/testis antigen 23 antibody
    • Cancer/testis antigen OY TES 1 antibody
    • Cancer/testis antigen OY-TES-1 antibody
    • CT23 antibody
    • FLJ51160 antibody
    • OY TES 1 antibody
    • Proacrosin binding protein sp32 antibody
    • Proacrosin-binding protein sp32 antibody
    • SP32 antibody
    see all

Images

  • ICC/IF image of ab64809 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab64809 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution

    Lane 1 : Extracts of 293 cells
    Lane 2 : Extracts of HepG2 cells
    Lane 3 : Extracts of Jurkat cells
    Lane 4 : Extracts of Jurkat cells with immunizing Peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Li X  et al. Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma. Oncol Lett 13:3080-3086 (2017). Read more (PubMed: 28529561) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
300000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (SKOV6 cells)
Specification
SKOV6 cells
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

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Verified customer

Submitted Mar 13 2014

Thank you for your enquiry. So I can complete the information regarding your testing discount promotion code, please let me know which institution you are working at. Thanks for the additional information!

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