Anti-Acrosin antibody (ab125362)

Overview

  • Product nameAnti-Acrosin antibodySee all Acrosin primary antibodies ...
  • Description
    Rabbit polyclonal to Acrosin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 231-280 (GKIDTCQGDS GGPLMCKDSK ESAYVVVGIT SWGVGCARAK RPGIYTATWP) of Human Acrosin protein (NP_001088).

  • Positive control
    • Human fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceAcrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
  • Database links
  • Alternative names
    • ACR antibody
    • Acrosin precursor antibody
    • ACRS antibody
    • Preproacrosin antibody
    • Proacrosin antibody
    see all

Anti-Acrosin antibody images

  • Anti-Acrosin antibody (ab125362) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 46 kDa

References for Anti-Acrosin antibody (ab125362)

ab125362 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

I am happy to provide discount code for others antib...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Yu-Ching Zachary

Verified customer

Submitted Aug 29 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"