Anti-Acrosin antibody (ab125362)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Acrosin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 231-280 (GKIDTCQGDS GGPLMCKDSK ESAYVVVGIT SWGVGCARAK RPGIYTATWP) of Human Acrosin protein (NP_001088).

  • Positive control
    • Human fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acr antibody
    • ACRO_HUMAN antibody
    • Acrosin heavy chain antibody
    • Acrosin precursor antibody
    • ACRS antibody
    • Preproacrosin antibody
    • Proacrosin antibody
    see all

Anti-Acrosin antibody images

  • Anti-Acrosin antibody (ab125362) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 46 kDa

References for Anti-Acrosin antibody (ab125362)

ab125362 has not yet been referenced specifically in any publications.

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The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

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Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (adult testis)
Specification
adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Aug 29 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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