Overview

  • Product nameAnti-ACSBG1 antibody [2B6]
    See all ACSBG1 primary antibodies
  • Description
    Mouse monoclonal [2B6] to ACSBG1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human ACSBG1 produced in HEK293T cells (NP_055977).

  • Positive control
    • ACSBG1 transfected HEK293T cell lysate, Human colon, kidney, liver, Adenocarcinoma of ovary, and pancreas tissues
  • General notesDilute in PBS (pH7.3) before use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab118154 was purified from mouse ascites fluids by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number2B6
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 81 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Also able to activate very long-chain fatty acids; however, the relevance of such activity is unclear in vivo. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids.
  • Tissue specificityExpressed primarily in brain. Expressed at lower level in testis and adrenal gland. Present in all regions of brain except pituitary.
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle. Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACBG1_HUMAN antibody
    • ACSBG 1 antibody
    • ACSBG1 antibody
    • Acyl CoA synthetase bubblegum family member 1 antibody
    • Acyl-CoA synthetase bubblegum family member 1 antibody
    • BG antibody
    • BG1 antibody
    • BGM antibody
    • Bubblegum antibody
    • FLJ30320 antibody
    • GR LACS antibody
    • GRLACS antibody
    • hBG1 antibody
    • hsBG antibody
    • hsBGM antibody
    • KIAA0631 antibody
    • Lipidosin antibody
    • Lipodisin antibody
    • Long chain fatty acid CoA ligase ACSBG1 antibody
    • Long-chain-fatty-acid--CoA ligase ACSBG1 antibody
    • LPD antibody
    • MGC14352 antibody
    • Ortholog of rodent gonadotropin regulated long chain acyl CoA synthetase antibody
    • Very long chain acyl CoA synthetase antibody
    see all

Anti-ACSBG1 antibody [2B6] images

  • Overlay histogram showing SH-SY5Y cells stained with ab118154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118154, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-ACSBG1 antibody [2B6] (ab118154) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ACSBG1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 81 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201652)
  • Immunohistochemical staining of paraffin-embedded Human colon tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human Adenocarcinoma of ovary tissue using ab118154 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab118154 at a dilution of 1/50

References for Anti-ACSBG1 antibody [2B6] (ab118154)

This product has been referenced in:
  • Naka-Kaneda H  et al. The miR-17/106-p38 axis is a key regulator of the neurogenic-to-gliogenic transition in developing neural stem/progenitor cells. Proc Natl Acad Sci U S A 111:1604-9 (2014). Read more (PubMed: 24474786) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab118154.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"