The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 81 kDa.
1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Able to activate long-chain fatty acids. Also able to activate very long-chain fatty acids; however, the relevance of such activity is unclear in vivo. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids.
Tissue specificityExpressed primarily in brain. Expressed at lower level in testis and adrenal gland. Present in all regions of brain except pituitary.
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
Overlay histogram showing SH-SY5Y cells stained with ab118154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118154, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-ACSBG1 antibody [2B6] (ab118154)
All lanes : Anti-ACSBG1 antibody [2B6] (ab118154) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ACSBG1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size : 81 kDa HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201652)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab118154 at a dilution of 1/50
References for Anti-ACSBG1 antibody [2B6] (ab118154)
This product has been referenced in:
Naka-Kaneda H et al. The miR-17/106-p38 axis is a key regulator of the neurogenic-to-gliogenic transition in developing neural stem/progenitor cells. Proc Natl Acad Sci U S A111:1604-9 (2014).
Read more (PubMed: 24474786) »