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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
Function Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
Tissue specificity Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
Sequence similarities Belongs to the ATP-dependent AMP-binding enzyme family.
Developmental stage Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
Cellular localization Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
Information by UniProt
ACS 1 antibody
ACSL 1 antibody
Anti-ACSL1 antibody images
Western blot - ACSL1 antibody (ab76702)
All lanes : Anti-ACSL1 antibody (ab76702) at 1 µg/ml Lane 1 : Human liver tissue lysate - total protein ( ab29889) Lane 2 : Human kidney tissue lysate - total protein ( ab30203) Lane 3 : Human heart tissue lysate - total protein ( ab29431) Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 78 kDa Observed band size : 78 kDa Additional bands at : 75 kDa (possible isoform). Exposure time : 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-ACSL1 antibody (ab76702)
ICC/IF image of ab76702 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76702, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ACSL1 antibody (ab76702)
This product has been referenced in:
et al. High-fructose diet downregulates long-chain acyl-CoA synthetase 3 expression in liver of hamsters via impairing LXR/RXR signaling pathway. J Lipid Res 54:1241-54 (2013).
Read more (PubMed: 23427282) »
et al. Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains. J Cell Biol 203:985-1001 (2013).
Read more (PubMed: 24368806) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"