Overview

  • Product name
  • Description
    Rabbit polyclonal to ACSL1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ACSL1.

    (Peptide available as ab90244.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Liver; Kidney; Heart.

Applications

Our Abpromise guarantee covers the use of ab76702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

Target

  • Function
    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
  • Tissue specificity
    Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
  • Sequence similarities
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Developmental stage
    Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
  • Cellular localization
    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS 1 antibody
    • ACS1 antibody
    • ACSL 1 antibody
    • ACSL1 antibody
    • ACSL1_HUMAN antibody
    • Acyl CoA synthetase 1 antibody
    • Acyl CoA synthetase long chain family member 1 antibody
    • Acyl-CoA synthetase 1 antibody
    • FACL 1 antibody
    • FACL 2 antibody
    • FACL1 antibody
    • FACL2 antibody
    • Fatty acid Coenzyme A ligase long chain 1 antibody
    • Fatty acid Coenzyme A ligase long chain 2 antibody
    • LACS 1 antibody
    • LACS 2 antibody
    • LACS antibody
    • LACS1 antibody
    • LACS2 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 1 antibody
    • Long chain acyl CoA synthetase 2 antibody
    • Long chain fatty acid CoA ligase 1 antibody
    • Long chain fatty acid CoA ligase 2 antibody
    • Long chain fatty acid coenzyme A ligase 1 antibody
    • Long-chain acyl-CoA synthetase 1 antibody
    • Long-chain acyl-CoA synthetase 2 antibody
    • Long-chain fatty acid-CoA ligase 2 antibody
    • Long-chain-fatty-acid--CoA ligase 1 antibody
    • Palmitoyl CoA ligase 1 antibody
    • Palmitoyl CoA ligase 2 antibody
    • Palmitoyl-CoA ligase 1 antibody
    • Palmitoyl-CoA ligase 2 antibody
    • Paltimoyl CoA ligase 1 antibody
    see all

Anti-ACSL1 antibody images

  • All lanes : Anti-ACSL1 antibody (ab76702) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 75 kDa (possible isoform).

    Exposure time : 3 minutes
  • ICC/IF image of ab76702 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76702, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ACSL1 antibody (ab76702)

This product has been referenced in:
  • Singh AB  et al. SREBP2 Activation Induces Hepatic Long-chain Acyl-CoA Synthetase 1 (ACSL1) Expression in Vivo and in Vitro through a Sterol Regulatory Element (SRE) Motif of the ACSL1 C-promoter. J Biol Chem 291:5373-84 (2016). Read more (PubMed: 26728456) »
  • Kan CF  et al. PPARd activation induces hepatic long-chain acyl-CoA synthetase 4 expression in vivo and in vitro. Biochim Biophys Acta 1851:577-87 (2015). Read more (PubMed: 25645621) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375)
Loading amount
20 µg
Specification
A375
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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