Overview

  • Product nameAnti-ACSL1 antibody
    See all ACSL1 primary antibodies
  • Description
    Rabbit polyclonal to ACSL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human ACSL1.

    (Peptide available as ab90245.)

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate and in the following human tissue lysates: Liver; Kidney; Heart.

Applications

Our Abpromise guarantee covers the use of ab89854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

Target

  • FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
  • Tissue specificityHighly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Developmental stageExpressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
  • Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS 1 antibody
    • ACS1 antibody
    • ACSL 1 antibody
    • ACSL1 antibody
    • ACSL1_HUMAN antibody
    • Acyl CoA synthetase 1 antibody
    • Acyl CoA synthetase long chain family member 1 antibody
    • Acyl-CoA synthetase 1 antibody
    • FACL 1 antibody
    • FACL 2 antibody
    • FACL1 antibody
    • FACL2 antibody
    • Fatty acid Coenzyme A ligase long chain 1 antibody
    • Fatty acid Coenzyme A ligase long chain 2 antibody
    • LACS 1 antibody
    • LACS 2 antibody
    • LACS antibody
    • LACS1 antibody
    • LACS2 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 1 antibody
    • Long chain acyl CoA synthetase 2 antibody
    • Long chain fatty acid CoA ligase 1 antibody
    • Long chain fatty acid CoA ligase 2 antibody
    • Long chain fatty acid coenzyme A ligase 1 antibody
    • Long-chain acyl-CoA synthetase 1 antibody
    • Long-chain acyl-CoA synthetase 2 antibody
    • Long-chain fatty acid-CoA ligase 2 antibody
    • Long-chain-fatty-acid--CoA ligase 1 antibody
    • Palmitoyl CoA ligase 1 antibody
    • Palmitoyl CoA ligase 2 antibody
    • Palmitoyl-CoA ligase 1 antibody
    • Palmitoyl-CoA ligase 2 antibody
    • Paltimoyl CoA ligase 1 antibody
    see all

Anti-ACSL1 antibody images

  • All lanes : Anti-ACSL1 antibody (ab89854) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 25 kDa,75 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-ACSL1 antibody (ab89854)

ab89854 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375)
Loading amount 20 µg
Specification A375
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"