Overview

  • Product nameAnti-ACSL3 antibody
    See all ACSL3 primary antibodies
  • Description
    Rabbit polyclonal to ACSL3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide: NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG , corresponding to N terminal amino acids 72-121 of Human ACSL3

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAcyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS3 antibody
    • Acsl3 antibody
    • ACSL3_HUMAN antibody
    • Acyl CoA synthetase long chain family member 3 antibody
    • FACL3 antibody
    • Fatty acid Coenzyme A ligase long chain 3 antibody
    • LACS 3 antibody
    • LACS3 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 3 antibody
    • Long chain fatty acid CoA ligase 3 antibody
    • Long-chain acyl-CoA synthetase 3 antibody
    • Long-chain-fatty-acid--CoA ligase 3 antibody
    • PRO2194 antibody
    see all

Anti-ACSL3 antibody images

  • Anti-ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage activated with iMtb. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333

References for Anti-ACSL3 antibody (ab80675)

ab80675 has not yet been referenced specifically in any publications.

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