Overview

  • Product nameAnti-ACSL5 antibody
    See all ACSL5 primary antibodies
  • Description
    Goat polyclonal to ACSL5
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RTQIDSLYEHIQD

    with a Cysteine residue linker, corresponding to amino acids 671-683 of Human ACSL5.

  • Positive control
    • Human small intestine tissue; Human spleen lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 76 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceACSL5 is an isozyme of the long chain fatty acid coenzyme A ligase family. All isozymes of this family convert free long chain fatty acids into fatty acyl CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. ACSL5 is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid induced glioma cell growth. Three transcript variants encoding two different isoforms have been found.
  • Cellular localizationMitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Database links
  • Alternative names
    • ACS2 antibody
    • ACS5 antibody
    • Acyl CoA synthetase 5 antibody
    • Acyl CoA synthetase long chain family member 5 antibody
    • EC 6.2.1.3 antibody
    • FACL5 antibody
    • FACL5 for fatty acid coenzyme A ligase 5 antibody
    • Fatty acid CoA ligase, long chain 5 antibody
    • Fatty acid coenzyme A ligase 5 antibody
    • Fatty acid Coenzyme A ligase long chain 5 antibody
    • LACS 5 antibody
    • Long chain acyl CoA synthetase 5 antibody
    • Long chain fatty acid CoA ligase 5 antibody
    • Long chain fatty acid coenzyme A ligase 5 antibody
    see all

Anti-ACSL5 antibody images

  • ab115526 at 2.5ug/ml staining ACSL5 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-ACSL5 antibody (ab115526) at 1 µg/ml + Human spleen lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 76 kDa

References for Anti-ACSL5 antibody (ab115526)

ab115526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"