Overview

  • Product nameAnti-ACSL5 antibody
    See all ACSL5 primary antibodies
  • Description
    Mouse monoclonal to ACSL5
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PQPVLPLLDL NNQSVGIEGG ARKGVSQKNN DLTSCCFSDA KTMYEVFQRG LAVSDNGPCL GYRKPNQPYR WLSYKQVSDR AEYLGSCLLH KGYKSS, corresponding to amino acids 91-187 of Human ACSL5

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceACSL5 is an isozyme of the long chain fatty acid coenzyme A ligase family. All isozymes of this family convert free long chain fatty acids into fatty acyl CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. ACSL5 is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid induced glioma cell growth. Three transcript variants encoding two different isoforms have been found.
  • Cellular localizationMitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Database links
  • Alternative names
    • ACS2 antibody
    • ACS5 antibody
    • Acyl CoA synthetase 5 antibody
    • Acyl CoA synthetase long chain family member 5 antibody
    • EC 6.2.1.3 antibody
    • FACL5 antibody
    • FACL5 for fatty acid coenzyme A ligase 5 antibody
    • Fatty acid CoA ligase, long chain 5 antibody
    • Fatty acid coenzyme A ligase 5 antibody
    • Fatty acid Coenzyme A ligase long chain 5 antibody
    • LACS 5 antibody
    • Long chain acyl CoA synthetase 5 antibody
    • Long chain fatty acid CoA ligase 5 antibody
    • Long chain fatty acid coenzyme A ligase 5 antibody
    see all

Anti-ACSL5 antibody images

  • Overlay histogram showing HepG2 cells stained with ab57210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57210, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ACSL5 antibody (ab57210) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
  • ACSL5 antibody (ab57210) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

References for Anti-ACSL5 antibody (ab57210)

This product has been referenced in:
  • Nchoutmboube JA  et al. Increased long chain acyl-Coa synthetase activity and fatty acid import is linked to membrane synthesis for development of picornavirus replication organelles. PLoS Pathog 9:e1003401 (2013). WB . Read more (PubMed: 23762027) »
  • Tripodi D  et al. Gene expression profiling in sinonasal adenocarcinoma. BMC Med Genomics 2:65 (2009). Read more (PubMed: 19903339) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The following publications have used the same clone that we sell as ab57210 ACSL5 antibody: 1. Gene expression profiling in sinonasal adenocarcinoma. Tripodi D, Quemener S, Renaudin K, Ferron C, Malard O, Guisle...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"