Overview

  • Product name
  • Description
    Rabbit polyclonal to ACSM1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: HHLPQFDTKV IIQTLLKYPI NHFWGVSSIY RMILQQDFTS IRFPALEHCY TGGEVVLPKD QEEWKRRTG, corresponding to amino acids 292-360 of Human ACSM1.

  • Positive control
    • Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab126551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase.
  • Sequence similarities
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACSM1 antibody
    • ACSM1_HUMAN antibody
    • Acyl-CoA synthetase medium-chain family member 1 antibody
    • Acyl-coenzyme A synthetase ACSM1 antibody
    • BUCS1 antibody
    • Butyrate--CoA ligase 1 antibody
    • Butyryl-coenzyme A synthetase 1 antibody
    • Lipoate-activating enzyme antibody
    • MACS1 antibody
    • Middle-chain acyl-CoA synthetase 1 antibody
    • mitochondrial antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab126551 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab126551, at 1/20 dilution, staining ACSM1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

ab126551 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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