Human Predicted to work with:
Recombinant fragment, corresponding to internal sequence amino acids 368-629 of Human ACSS1 (BC039261).
Human fetal brain lysate
FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
FunctionImportant for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
Post-translational modificationsReversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.