Overview

  • Product nameAnti-ACSS1 antibody
    See all ACSS1 primary antibodies
  • Description
    Rabbit polyclonal to ACSS1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 368-629 of Human ACSS1 (BC039261).

  • Positive control
    • Human fetal brain lysate

Properties

  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab101570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

Target

  • FunctionImportant for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Post-translational
    modifications
    Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAS2L antibody
    • AceCS2 antibody
    • AceCS2L antibody
    • Acetate CoA ligase 2 antibody
    • Acetate--CoA ligase 2 antibody
    • Acetyl CoA synthetase 2 antibody
    • Acetyl Coenzyme A synthetase 2 (AMP forming) like antibody
    • Acetyl coenzyme A synthetase 2 like, mitochondrial antibody
    • Acetyl-CoA synthetase 2 antibody
    • Acetyl-coenzyme A synthetase 2-like, mitochondrial antibody
    • ACS2L_HUMAN antibody
    • Acss1 antibody
    • Acyl CoA synthetase short chain family member 1 antibody
    • Acyl-CoA synthetase short-chain family member 1 antibody
    • FLJ45659 antibody
    • KIAA1846 antibody
    • MGC33843 antibody
    see all

Anti-ACSS1 antibody images

  • Anti-ACSS1 antibody (ab101570) at 1/500 dilution + Human fetal brain lysate

    Predicted band size : 75 kDa
    Observed band size : 75 kDa

References for Anti-ACSS1 antibody (ab101570)

ab101570 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Rat Cell lysate - whole cell (Ovarian granulosa cells)
Specification Ovarian granulosa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 12 2013

Western blot

Excellent
Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"