• Product nameAnti-ACTH antibody [57]
    See all ACTH primary antibodies
  • Description
    Mouse monoclonal [57] to ACTH
  • SpecificitySpecific for Synacthen (1-24 ACTH) and has no cross reaction with CLIP(ACTH 17-39). Positive with rat N-terminal ACTH peptide. Reacts <1% with insulin, KLH and BSA. Cross reacts with rat N-terminal ACTH.
  • Tested applicationsSuitable for: IHC-P, ICC, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    ACTH Hormone (N terminal) conjugated to KLH.



Our Abpromise guarantee covers the use of ab20358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    Reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen.
    IHC-P: Use at a concentration of 1 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • ACTH antibody
      • Adrenocorticotropic hormone antibody
      • Adrenocorticotropin antibody
      • Alpha melanocyte stimulating hormone antibody
      • alpha-MSH antibody
      • alphaMSH antibody
      • Beta LPH antibody
      • Beta melanocyte stimulating hormone antibody
      • Beta-endorphin antibody
      • beta-MSH antibody
      • CLIP antibody
      • Corticotropin antibody
      • Corticotropin lipotropin antibody
      • Corticotropin-like intermediary peptide antibody
      • Gamma LPH antibody
      • gamma-MSH antibody
      • Lipotropin beta antibody
      • Lipotropin gamma antibody
      • Lipotropin, included antibody
      • LPH antibody
      • Melanocyte-stimulating hormone, included antibody
      • Melanotropin alpha antibody
      • Melanotropin beta antibody
      • Melanotropin gamma antibody
      • Melanotropin, included antibody
      • Met-enkephalin antibody
      • MSH antibody
      • NPP antibody
      • Opiomelanocortin prepropeptide antibody
      • POC antibody
      • POMC antibody
      • Pomc-1 antibody
      • Pomc1 antibody
      • Pomc2 antibody
      • Pro ACTH endorphin antibody
      • Pro opiomelanocortin antibody
      • Pro-opiomelanocortin-alpha antibody
      • Proopiomelanocortin antibody
      • Proopiomelanocortin preproprotein antibody
      • Tetracosactide antibody
      see all

    Anti-ACTH antibody [57] images

    • Ab20358 staining human normal pituitary gland. Staining is localised to the cytoplasm and is secreted.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References for Anti-ACTH antibody [57] (ab20358)

    ab20358 has not yet been referenced specifically in any publications.

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