• Product nameAnti-ACTH antibody
    See all ACTH primary antibodies
  • Description
    Rabbit polyclonal to ACTH
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Goat, Chicken, Guinea pig, Cow, Dog, Chimpanzee, Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to ACTH aa 138-176.




Our Abpromise guarantee covers the use of ab74976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 29 kDa.
IP 1/200.


  • RelevanceACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • Alpha melanocyte stimulating hormone antibody
    • alpha-MSH antibody
    • alphaMSH antibody
    • Beta LPH antibody
    • Beta melanocyte stimulating hormone antibody
    • Beta-endorphin antibody
    • beta-MSH antibody
    • CLIP antibody
    • Corticotropin antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-like intermediary peptide antibody
    • Gamma LPH antibody
    • gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • Lipotropin, included antibody
    • LPH antibody
    • Melanocyte-stimulating hormone, included antibody
    • Melanotropin alpha antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • Melanotropin, included antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • Opiomelanocortin prepropeptide antibody
    • POC antibody
    • POMC antibody
    • Pomc-1 antibody
    • Pomc1 antibody
    • Pomc2 antibody
    • Pro ACTH endorphin antibody
    • Pro opiomelanocortin antibody
    • Pro-opiomelanocortin-alpha antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    • Tetracosactide antibody
    see all

Anti-ACTH antibody images

  • ab74976 staining ACTH in P0 Pitx2-Cre/Dicer1 mutant mice pituitary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Samples were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin wax. Sections were cut (7 µm) and stained with H&E. Immunohistochemistry was done on 7-µm paraffin sections stained by an indirect immunoperoxidase method. Peroxidase activity was visualized with AEC stain kit. ab74976 diluted 1/200. Secondary antibodies conjugated with biotin were used at 1/150 dilution.

References for Anti-ACTH antibody (ab74976)

This product has been referenced in:
  • Holst B  et al. PICK1 deficiency impairs secretory vesicle biogenesis and leads to growth retardation and decreased glucose tolerance. PLoS Biol 11:e1001542 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23630454) »
  • Füchsl AM  et al. Mechanisms underlying the increased plasma ACTH levels in chronic psychosocially stressed male mice. PLoS One 8:e84161 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24376791) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Pig Purified protein (brain)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10 µg
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

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Verified customer

Submitted Oct 27 2015

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Unfortunately we do not have any images for sharing we however can provide 100% image discount, if customer tests the antibody and submit an image in the form of Abreview. Please let me know if you want an Abtrial...

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You can use any antibody however we will not be able to provide details about the purity of isolated protein.

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