Anti-Actin [1A4+5C5] antibody (ab17146)

Overview

  • Product nameAnti-Actin [1A4+5C5] antibodySee all Actin primary antibodies ...
  • Description
    Mouse monoclonal [1A4+5C5] to Actin
  • Specificityab17146 is useful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma. The isotypes for ab17146 are IgG2a kappa for clone 1A4; IgM for clone 5C5
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Guinea pig, Cow, Human, Snake
  • Immunogen

    Other Immunogen Type corresponding to Actin. BALB/C mice were injected with N-terminal decapeptide of α-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5.

  • Positive control
    • Skeletal muscle leiomyoma

Properties

Applications

Our Abpromise guarantee covers the use of ab17146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/25 - 1/50.

ABC method.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • alpha Actin 1 antibody
    • alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Anti-Actin [1A4+5C5] antibody images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human uterus leiomyoma tissue, using ab17146 at 1/25 dilution.

References for Anti-Actin [1A4+5C5] antibody (ab17146)

ab17146 has not yet been referenced specifically in any publications.

Product Wall

We can confirm that alpha cardiac striated muscle actin is also called sarcomeric actin. For example, Ab7799 reacts with alpha cardiac striated muscle (sarcomeric) and alpha skeletal muscle. Ab7817 reacts with alpha smooth muscle, does not react with...

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