Overview

  • Product nameAnti-Actin [2Q1055] antibodySee all Actin primary antibodies ...
  • Description
    Mouse monoclonal [2Q1055] to Actin
  • Specificityab14128 recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ab14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, ab14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA.
  • Tested applicationsWB, ICC more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Donkey, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Saccharomyces cerevisiae, Tetrahymena sp., Bird, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Monkey, a wide range of other species, all Mammals, Escherichia coli , Goose, Macaque Monkey, Frog, Equus, Rooster
  • Immunogen

    Recombinant fragment corresponding to Chicken Actin aa 50-70 (N terminal). The immuogen is purified chicken gizzard actin. This is predominantly smooth muscle gamma-actin. Is a pan-actin which recognizes all actin isoforms.
    Sequence: KDSYVGDEAQ SKRGILTLKY
    Database link: P63270

  • EpitopeThe epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
  • Positive control
    • This antibody gave a positive signal in both Human Skeletal Muscle and Heart tissue lysates as well as the following whole cell lysates: A431; HEK293; MCF7; HeLa; MDA MB 231.
  • General notesFor maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab14128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 20 µg/ml. Predicted molecular weight: 43 kDa.

On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kDa protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.

ICC 1/500.

Methanol fixed HeLa and NIH/3T3 cells

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • alpha Actin 1 antibody
    • alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Anti-Actin [2Q1055] antibody images

  • All lanes : Anti-Actin [2Q1055] antibody (ab14128) at 1/1000 dilution

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-231 (Breast Carcinoma cell line)
    Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
    Lane 7 : Heart (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab14128 overnight at 4°C. Antibody binding was detected using ab175783 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

References for Anti-Actin [2Q1055] antibody (ab14128)

This product has been referenced in:
  • Christiansen VJ  et al. Targeting inhibition of fibroblast activation protein-a and prolyl oligopeptidase activities on cells common to metastatic tumor microenvironments. Neoplasia 15:348-58 (2013). WB ; Human . Read more (PubMed: 23555181) »
  • Oven I  et al. Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate immune responses. Vet Res 44:99 (2013). Read more (PubMed: 24134665) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Chlamydomonas reinhardtii Cell lysate - whole cell (Chlamydomonas reinhardtii)
Loading amount 20 µg
Specification Chlamydomonas reinhardtii
Treatment Various concentrations of calcium (0M to 10^-3M)
Gel Running Conditions Non-reduced Denaturing (12% gradient gel (polyacrylamide))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 23°C
Username

Caroline Lam

Verified customer

Submitted May 10 2013

Thank you for your reply.
We have beta actin antibodies that would fulfill all your criteria:
ab8227 (http://www.abcam.com/beta-Actin-antibody-Loading-Control-ab8227.html) - in case beta actin would be acceptable for your experiments.
Or...

Read More

Thank you for your reply.
If the word "actin" is entered into the search box on our homepage (top right corner), you will obtain entries for 21 actin antibodies, 9 actin-loading control antibodies, etc.
We do not have a rabbit anti-actin anti...

Read More
Application ELISA
Sample Human Cell (Fibroblast)
Specification Fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Feb 11 2009

Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 8000 cells
Specification MEF
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2009

Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - whole cell (cells were grown to 3.5x10 7 cells/ml)
Loading amount 175000 cells
Specification cells were grown to 3.5x10 7 cells/ml
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2007

Thank you for your enquiry. The list of species this antibody has been tested in is quite impressive. The description of the antibody claims it is "pan-actin" due the highly conserved nature of actin. The antibody has indeed been tested specifica...

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