Anti-Actin [2Q1055] antibody (ab14128)
- Product nameAnti-Actin [2Q1055] antibodySee all Actin primary antibodies ...
- DescriptionMouse monoclonal [2Q1055] to Actin
- Specificityab14128 recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ab14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, ab14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA.
- Tested applicationsWB, ICC more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Donkey, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Saccharomyces cerevisiae, Tetrahymena sp., Bird, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Monkey, a wide range of other species, all Mammals, Escherichia coli , Goose, Macaque Monkey, Frog, Equus, Rooster
Recombinant fragment corresponding to Chicken Actin (N terminal).
- EpitopeThe epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
- General notesFor maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: Tris glycine, 0.88% Sodium chloride
- Concentration information loading...
- PurityProtein G purified
- Purification notesPurified by Protein G affinity chromatography.
- Clone number2Q1055
- Light chain typekappa
Our Abpromise guarantee covers the use of ab14128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 20 µg/ml. Predicted molecular weight: 43 kDa.
On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kDa protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.
Methanol fixed HeLa and NIH/3T3 cells
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
- Entrez Gene: 421534 Chicken
- Entrez Gene: 281592 Cow
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100154254 Pig
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
- SwissProt: P68138 Cow
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
- SwissProt: P68137 Pig
- SwissProt: P68135 Rabbit
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 214950 Mouse
- Unigene: 82732 Rat
- ACTA antibody
- ACTA1 antibody
- Actin alpha skeletal muscle antibody
- Actin antibody
- actin, alpha 1, skeletal muscle 1 antibody
- actin, alpha 1, skeletal muscle antibody
- Actin, alpha skeletal muscle antibody
- actina antibody
- actine antibody
- ACTS_HUMAN antibody
- aktin antibody
- alpha Actin 1 antibody
- alpha skeletal muscle Actin antibody
- alpha skeletal muscle antibody
- alpha-actin antibody
- Alpha-actin-1 antibody
- ASMA antibody
- CFTD antibody
- CFTD1 antibody
- CFTDM antibody
- MPFD antibody
- NEM1 antibody
- NEM2 antibody
- NEM3 antibody
- nemaline myopathy type 3 antibody
References for Anti-Actin [2Q1055] antibody (ab14128)
This product has been referenced in:
- Christiansen VJ et al. Targeting inhibition of fibroblast activation protein-a and prolyl oligopeptidase activities on cells common to metastatic tumor microenvironments. Neoplasia 15:348-58 (2013). WB ; Human . Read more (PubMed: 23555181) »
- Siegel MP et al. Reduced coupling of oxidative phosphorylation in vivo precedes electron transport chain defects due to mild oxidative stress in mice. PLoS One 6:e26963 (2011). WB ; Mouse . Read more (PubMed: 22132085) »