Overview

  • Product name
    Anti-Actin antibody [AC-40]
    See all Actin primary antibodies
  • Description
    Mouse monoclonal [AC-40] to Actin
  • Host species
    Mouse
  • Tested applications
    Suitable for: Dot blot, WB, ELISA, IHC-FoFr, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail
  • Immunogen

    Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
    Sequence:

    SGPSIVHRKCF

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope
    Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab219733 as a replacement.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

Our Abpromise guarantee covers the use of ab11003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts.

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [AC-40] (ab11003)
    Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [AC-40] (ab11003)This image is courtesy of an anonymous Abreview

    ab11003 staining Actin in Human SW480 cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 2 hours at 25°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 568 (red) used at a 1/100 dilution.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] (ab11003)
    Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] (ab11003)

    Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.

  • ELISA - Anti-Actin antibody [AC-40] (ab11003)
    ELISA - Anti-Actin antibody [AC-40] (ab11003)This image is courtesy of an anonymous Abreview

    ab11003 staining Human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The  primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody.

    See Abreview

References

This product has been referenced in:
  • Nguyen AM  et al. The primary cilium is a self-adaptable, integrating nexus for mechanical stimuli and cellular signaling. Biol Open 4:1733-8 (2015). WB ; Mouse . Read more (PubMed: 26603473) »
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Rat . Read more (PubMed: 25794768) »

See all 26 Publications for this product

Publishing research using ab11003? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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11 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Actin antibody [AC-40]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse kidney (IMCD))
Loading amount
7.5 µg
Specification
Mouse kidney (IMCD)
Gel Running Conditions
Reduced Denaturing (4 -12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 24 2012

I have a problem with the antibody ab6276. I try to develop an indirect ELISA with this antibody, but do not get any result. This is the procedure, I've performed: - Coating of ELISA plate (Costar high bind) with actin (rabbit skeletal muscle, Cytoskel...

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Thank you for contacting us.

I would not recommend adding SDS or a reducing agent to the sample prior to coating, as this may interfere with the coating efficiency. It is possible that by boiling the protein for 5-10 minutes, the heat denatur...

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Could you please advise the lot & expiry for the new lot of ab11003?

The new lot of ab11003 is currently in transit from the production lab to our stock and there is no lot number allocated yet, but I can confirm that this new lot is different from the lot your customer has received originally.

I will send you...

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Our customer would like to take back the same item, AB11003. However, they would like to have a another lot other thanGR2058. Please revert if this is acceptable.

Thank you for your reply.

Unfortunately we do not have any other lot of ab11003 in stock.

Would your customer be interested in trying an alternative anti-actin from the following list (excluding ab11003 of course) : http://www.abcam...

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Resend
1) Abcam product code ab11003

2) Abcam order reference number or product batch number

Cat no:AB11003

Lot no.: GR2058-9

3) Description of the problem: no band / high background (many non-specific b...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem.

I have looked through your protocol, which looks fine to ...

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One of our customer purchased the above item and had faced difficulties with working on the anti body. I have attached pictures, displaying the results of the experiment.

Thank you for contacting us.

I am sorry to hear your customeris experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody [AC-40]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SW480)
Specification
SW480
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted May 24 2010

Western blot abreview for Anti-Actin antibody [AC-40]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
8000 cells
Specification
MEF
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Jan 22 2009

ELISA abreview for Anti-Actin antibody [AC-40]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Fibroblast)
Specification
Fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Sandwich (Capture)
Read More
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Abcam user community

Verified customer

Submitted Jan 20 2009

Our end user want to use loading control. but a8226 data sheet describe that " Does not cross-react with adult cardiac, smooth, or skeletal muscle actin. " The sample of end user is myoblast, so they wonder they can use this antibody. And another ...

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Thank you for your enquiry. Ab8226 does not cross-react with ADULT cardiac, smooth, or skeletal muscle actin. Myoblasts are immature muscle cells and looking through the literature it does seem that some people use actin as a loading control. Your cust...

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