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ELISA - Actin antibody [AC-40] (ab11003)
![ELISA - Actin antibody [AC-40] (ab11003)](/ps/datasheet/images/ab11003_review_13193.jpg)
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Product Name
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Actin antibody [AC-40]
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See all Actin antibodies (21)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [AC-40] to Actin
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Immunogen
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Synthetic peptide: SGPSIVHRKCF, corresponding to C terminal amino acids 365-375 of Human Actin, attached to Multiple Antigen Peptide (MAP) backbone.
BLAST 'SGPSIVHRKCF' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Carp, Chk, Cow, Dog, Goat, Gpig, Hm, Pig, Rb, Shp
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Specificity
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The antibody does not react with the epitope on the N-terminal end of actin.
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Epitope
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Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
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Tested applications
(see key)
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Dot, ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IHC-F: Use at an assay dependent dilution.
IF: 1/2000 determined by indirect immunofluorescent staining of cultured human or chicken fibroblasts.
IHC-P: Use at an assay dependent dilution. Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel’s or Bouin’s solution may also be used as fixatives.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/500 using cultured human or chicken fibroblast extract. Predicted molecular weight: 42 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
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Actin antibody [AC-40] (ab11003) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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AC-40
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Isotype
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IgG2a
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Purity
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Ascites
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Raw Ascites Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Actin antibody [AC-40] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Actin antibody [AC-40] images, references, products related to ab11003 and other tools.
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Actin antibody [AC-40] images:
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 ELISA - Actin antibody [AC-40] (ab11003)
ab11003 staining human fibroblast cells by ELISA (Sandwich-capture). Cells were blocked in 1% BSA for 1 hour at 25°C. The primary antibody ab11003 (Capture antibody) was diluted 1/3000 and incubated with sample for 12 hours at 4°C. ab5694 was used as secondary and a HRP conjugated goat polyclonal to mouse, diluted 1/1000 was used as detection antibody. This image is courtesy of an anonymous Abreview
See Abreview
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References for Actin antibody [AC-40] (ab11003)
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This product has been used in: (two most recent references)
Macovei A et al. Hepatitis B virus requires intact caveolin-1 function for productive infection in HepaRG cells. J Virol 84:243-53 (2010). WB; Human. PubMed: 19846513
Shepard BD et al. Chronic ethanol consumption induces global hepatic protein hyperacetylation. Alcohol Clin Exp Res 34:280-91 (2010). WB; Rat. PubMed: 19951295
See all 6 publication references for this product.
If you publish research using ab11003 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Actin
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Actin antibody [AC-40] - more information
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Customer reviews (feedback) regarding Actin antibody [AC-40] |
Customer FAQs regarding Actin antibody [AC-40] |
Protocols for Actin antibody [AC-40] |
Price and availability of products related to Actin antibody [AC-40] |
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Actin antibody [AC-40] - related products:
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Actin antibody [AC-40] - other tools:
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Contact Abcam with a Technical Enquiry about Actin antibody [AC-40] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![ELISA - Actin antibody [AC-40] (ab11003)](/index.html?datasheet=11003#ab11003_review_13193.jpg)
