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Chicken gizzard Actin
This antibody is highly recommended for monitoring total protein load on Western blots.
Our Abpromise guarantee covers the use of ab76548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 42 kDa.|
|IP||Use at 2 µg/mg of lysate. Use Protein G.|
|IHC-P||1/50. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 - 20 minutes followed by cooling at room temperature for 20 minutes.|
ab76548 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab76548 at 10µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-mouse DyLight® 488 (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) Hek293, HepG2, and MCF-7 cells at 10ug/ml.
ab76548 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"