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- Proteins and Peptides
- Secondary antibodies
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Product name
Anti-Actin antibody - Loading Control
See all Actin products (22) ...
Description
Rabbit polyclonal to Actin - Loading Control
Specificity
This antibody recognises beta and gamma actin in human samples. It probably also recognises all the other known forms of human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish
Predicted to work with
Rabbit, Saccharomyces cerevisiae
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin.
(Peptide available as ab137 71.)
Positive control
HeLa whole cell lysate or mouse brain lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> RIP
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Tropomyosin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Troponin
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Block in 5% BSA. Blocking in milk significantly reduces the signal.
Application notes
Is unsuitable for or ICC/IF.
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similarities
Belongs to the actin family.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP68133
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- alpha skeletal muscle antibody
- ACTA antibody
- ACTA1 antibody
see all
Database links
- Entrez Gene: 280979 Cow
- Entrez Gene: 60 Human
- Entrez Gene: 71 Human
- Entrez Gene: 70 Human
- Entrez Gene: 72 Human
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100009272 Rabbit
see all
Anti-Actin antibody - Loading Control images:
Western blot - Actin antibody (ab1801)
All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Rat brain
Lane 3 : Mouse brain
Lane 4 : 3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 1 minute
Western blot - Actin antibody (ab1801)
All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution
Lane 1 : Whole cell lysates prepared from HUVEC cells
Lane 2 : Whole cell lysates prepared from HUVEC cells
Lane 3 : Whole cell lysates prepared from HUVEC cells
Lane 4 : Whole cell lysates prepared from HUVEC cells
Lysates/proteins at 15 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Western blot - Actin antibody - Loading Control (ab1801)
Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution
Secondary
An HRP conjugated donkey anti rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Western blot
Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Anti-Actin antibody - Loading Control (ab1801)
This product has been referenced in:
- Spencer VA et al. Depletion of nuclear actin is a key mediator of quiescence in epithelial cells. J Cell Sci 124:123-32 (2011). ICC, IF; Mouse.Read more (PubMed: 21172822) »
- Chen AY et al. The small 11 kDa nonstructural protein of human parvovirus B19 plays a key role in inducing apoptosis during B19 virus infection of primary erythroid progenitor cells. Blood 115:1070-80 (2010). WB; Human.Read more (PubMed: 19861680) »
See all 10 publications for this product
Publishing research using ab1801? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"