Anti-Actin Regulatory Protein CAPG antibody (ab155688)

Overview

  • Product name
    Anti-Actin Regulatory Protein CAPG antibody
    See all Actin Regulatory Protein CAPG primary antibodies
  • Description
    Rabbit polyclonal to Actin Regulatory Protein CAPG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 157-333 of Human Actin Regulatory Protein CAPG (UniProt: P40121).

  • Positive control
    • A549, HeLa, HepG2, HCT116 and NIH 3T3 whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity
    Macrophages and macrophage-like cells.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin capping protein GCAP39 antibody
    • Actin regulatory protein CAP G antibody
    • Actin regulatory protein CAP-G antibody
    • AFCP antibody
    • Capg antibody
    • CAPG_HUMAN antibody
    • Capping actin protein gelsolin like antibody
    • Capping protein (actin filament) gelsolin like antibody
    • Capping protein gelsolin like antibody
    • Epididymis secretory protein Li 66 antibody
    • gCap39 antibody
    • Gelsolin like capping protein antibody
    • HEL S 66 antibody
    • Macrophage capping protein antibody
    • Macrophage-capping protein antibody
    • mbh1 antibody
    • MCP antibody
    • Myc basic motif homolog 1 antibody
    see all

Images

  • Anti-Actin Regulatory Protein CAPG antibody (ab155688) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Anti-Actin Regulatory Protein CAPG antibody (ab155688) at 1/2000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Actin Regulatory Protein CAPG with ab155688 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling Actin Regulatory Protein CAPG with ab155688 at 1/500 dilution.

References

This product has been referenced in:
  • Kumar A  et al. Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation. Mol Cell Endocrinol 404:91-101 (2015). ICC/IF ; Rat . Read more (PubMed: 25637714) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Rat Cell (Testis sections)
Specification
Testis sections
Permeabilization
Yes - 0.1% Tween20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 16 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Tissue lysate - whole (testis)
Specification
testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Anita Kumar

Verified customer

Submitted Feb 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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