Anti-Actin Regulatory Protein CAPG antibody (ab188112)

Overview

  • Product name
    Anti-Actin Regulatory Protein CAPG antibody
    See all Actin Regulatory Protein CAPG primary antibodies
  • Description
    Goat polyclonal to Actin Regulatory Protein CAPG
  • Host species
    Goat
  • Specificity
    ab188112 is expected to recognize isoform 1 (NP_001738.2) only.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Actin Regulatory Protein CAPG aa 205-217 (internal sequence) (Cysteine residue). (NP_001738.2).
    Sequence:

    KAQVEIVTDGEEP


    Database link: P40121

  • Positive control
    • WB: U937, human and pig spleen lysates, and human peripheral blood lymphocytes lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab188112 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function
    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity
    Macrophages and macrophage-like cells.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin capping protein GCAP39 antibody
    • Actin regulatory protein CAP G antibody
    • Actin regulatory protein CAP-G antibody
    • AFCP antibody
    • Capg antibody
    • CAPG_HUMAN antibody
    • Capping actin protein gelsolin like antibody
    • Capping protein (actin filament) gelsolin like antibody
    • Capping protein gelsolin like antibody
    • Epididymis secretory protein Li 66 antibody
    • gCap39 antibody
    • Gelsolin like capping protein antibody
    • HEL S 66 antibody
    • Macrophage capping protein antibody
    • Macrophage-capping protein antibody
    • mbh1 antibody
    • MCP antibody
    • Myc basic motif homolog 1 antibody
    see all

Images

  • All lanes : Anti-Actin Regulatory Protein CAPG antibody (ab188112) at 0.3 µg/ml (Incubation Time: 1 hour)

    Lane 1 : U937 cell lysate (in RIPA buffer)
    Lane 2 : Human spleen lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    Primary incubation was 1 hour.

  • All lanes : Anti-Actin Regulatory Protein CAPG antibody (ab188112) at 0.1 µg/ml (Incubation Time: 1 hour)

    Lane 1 : Mouse spleen lysate (in RIPA buffer)
    Lane 2 : Rat spleen lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    Primary incubation was 1 hour.

  • Anti-Actin Regulatory Protein CAPG antibody (ab188112) at 0.3 µg/ml + Pig spleen lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    Primary incubation was 1 hour.

  • Anti-Actin Regulatory Protein CAPG antibody (ab188112) at 0.3 µg/ml + Human peripheral blood lymphocytes lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa



    Primary incubation was 1 hour.

References

ab188112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188112.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up