Anti-activated Notch1 antibody (ab52301)
Key features and details
- Rabbit polyclonal to activated Notch1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-activated Notch1 antibody -
Description
Rabbit polyclonal to activated Notch1 -
Host species
Rabbit -
Specificity
ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human activated Notch1 aa 1700-1800.
Database link: P46531 -
General notes
ab52301 is cleaved at Val 1744
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
PBS without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa). |
Target
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Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. -
Tissue specificity
In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. -
Involvement in disease
Defects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome. -
Sequence similarities
Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
Post-translational
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Phosphorylated.
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.
Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD. -
Cellular localization
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4851 Human
- Entrez Gene: 18128 Mouse
- Omim: 190198 Human
- SwissProt: P46531 Human
- SwissProt: Q01705 Mouse
- Unigene: 495473 Human
- Unigene: 290610 Mouse
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Alternative names
- hN1 antibody
- Neurogenic locus Notch homolog protein 1 antibody
- NICD antibody
see all
Images
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All lanes : Anti-activated Notch1 antibody (ab52301) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour)
Lane 2 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour) with immunising peptide
Predicted band size: 272 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (48)
ab52301 has been referenced in 48 publications.
- Jiang W et al. Critical Role of Notch-1 in Mechanistic Target of Rapamycin Hyperactivity and Vascular Inflammation in Patients With Takayasu Arteritis. Arthritis Rheumatol 74:1235-1244 (2022). PubMed: 35212196
- Gao Y et al. Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4. Front Pharmacol 13:797805 (2022). PubMed: 35846998
- Liu B et al. Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes. Stem Cells Int 2021:8859071 (2021). PubMed: 33897781
- Tang Y & Cao Y SOX10 Knockdown Inhibits Melanoma Cell Proliferation via Notch Signaling Pathway. Cancer Manag Res 13:7225-7234 (2021). PubMed: 34557039
- Huang S et al. LncRNA FEZF1-AS1 promotes non-small lung cancer cell migration and invasion through the up-regulation of NOTCH1 by serving as a sponge of miR-34a. BMC Pulm Med 20:110 (2020). PubMed: 32349744