Anti-active + pro Caspase 3 antibody [31A1067] (ab13585)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-active + pro Caspase 3 antibody [31A1067](ab13585)

IHC image of ab13585 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13585, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  Flow Cytometry-Anti-active + pro Caspase 3 antibody [31A1067](ab13585)

Overlay histogram showing A549 cells stained with ab13585 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13585, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab913530, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.