Overview
Product nameactive Caspase 3 peptideSee all active Caspase 3 proteins and peptides ...
Protein descriptionSynthetic peptide derived from within residues 150 - 250 of Human active Caspase 3.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab13847 - active Caspase 3 antibody and ab77973 - active Caspase 3 antibody.
Protein length277 amino acids
Expression hostCHO
Properties
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferInformation available upon request.
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab13848
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
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Application notesBL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab13847.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
APOPAINApopain precursorApopain precursor
CASP3CASP3Caspase 3 apoptosis related cysteine proteaseCaspase 3 apoptosis related cysteine proteaseCaspase3CPP 32CPP 32CPP32CPP32BCPP32BCysteine protease CPP32Cysteine protease CPP32Human cysteine protease CPP32 isoform alpha mRNA complete cdsHuman cysteine protease CPP32 isoform alpha mRNA complete cdsICE3ICE3LICELICEPARP cleavage proteasePARP cleavage proteaseSCA 1SCA1SREBP cleavage activity 1SREBP cleavage activity 1YamaYama proteinYama protein
see all
RelevanceThe caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Cellular localizationCytoplasmic
References for active Caspase 3 peptide (ab13848)
ab13848
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
