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active Caspase 3 protein (Active) (ab52101)

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Overview

  • Product nameactive Caspase 3 protein (Active)See all active Caspase 3 proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human)
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activitySPECIFIC ACTIVITY: 300,000-400,000 units/mg UNIT DEFINITION: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Additional notesThe lyophilized caspase-3 is stable for 1 year at –70oC The recombinant active human Caspase 3 spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab52101 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. We recommend using 1 unit/assay for analyzing caspase activity.

    Protein info

    • Alternative names
        CASP 3Caspase 3CPP 32
        CPP32BCysteine protease CPP32PARP cleavage proteaseSCA1SREBP cleavage activity 1Yama
      see all
  • RelevanceThe caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
  • Cellular localizationCytoplasmic

    • References for active Caspase 3 protein (Active) (ab52101)

    ab52101 has not yet been referenced specifically in any publications.

    Publishing research using ab52101 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab52101 Submit a Question for ab52101

    Displaying 1 - 10 of 11 results for Abreviews and Q&A

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    How much protein is provided with the active Caspase 3 protein (Active) (ab52101)

    The active Caspase 3 protein (Active), ab52101, is provided at the equivalent of 100 units. There are between 300,000-400,000 units/mg. Therefore a total of between 0.25-0.33 ug of protein is provide per vial.

    Description of the problem (high background, wrong band size, more bands, no band etc.)


    No band shown. In femto substrate high background.


    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc....

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Caspase 3 antibody [4H334] (ab17819) and active Caspase 3 protein (Active) (ab52101) .
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    What's the concentration?

    Thank you again for your call.

    Unfortunately, the total concentration of this protein is proprietary information. We sell these by the number of units of enzyme activity totally present in each vial. Therefore in case of ab52101, there would be ...

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    Custumer is looking for a product which can detect both uncleaved and cleaved Caspase3

    Thank you for contacting us.

    I would suggest to use ab17819 Anti-Caspase 3 antibody [4H334]

    http://www.abcam.com/index.html?datasheet=17819 (or use the following: http://www.abcam.com/index.html?datasheet=17819).

    Even if the...

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    Hello,

    Following our discussion over the phone previously, I forward you the list of the antibodies we used for our study.
    For your understanding, we are looking to investigate the Active caspase 3 and PLA2G1B in the canine population. The...

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    Thank you for providing that information and sorry for the delay in getting back to you.
    The information provided has been very useful and I will look into whether these antibodies would be likely to detect the protein and what may be contributing to...

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    customer can detect the pro-Caspace 3 and cleaved form as well, but notour protein! Used a biosensor to detced. Collegue in USA got same results.
    antibody is from Biolegend: 622704

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    I ordered an active caspase3 protein ( Active) (ab52101). And I would like to know after I reconstruct the product with PBS solution, what components I have? Procaspase-3 ? Large unit of caspase-3 ( 17KDa) ? or small unit of caspase-3 ( 12KDa)?

    You have received the recombinant full length protein: processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme.

    Purchased the protein and reconstituted as recommended, used the protein successfully during first experiment. Stored the protein as directed, but when went back to use it 2 weeks later, the protein was not active. Have tried a total 4 times, using varyin...

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    Thank you for calling Abcam earlier today.

    I am sorry about the issues you have been having with the Caspase 3 protein. As we discussed, I have requested that you be sent (via Cedarlane) a free of charge replacement for the protein.

    If...

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    We need some active caspase 3 recombinant protein and found your company sell it. Your catalogue number is ab52101. I can not find the exact concentration of the protein and we need the activation procession. Would you please provide some information abou...

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    Thank you for contacting Abcam.

    I have emailed the lab about your enquiry and below is their response:

    This product is not sold by concentration. It is sold by Units. The recombinant active human caspase-3 expressed in E coli spontaneo...

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    Our lab want to purchase caspase 1 (ab39901) and caspase 3 (ab52101) for
    my project. We have one question regarding these products. We want to know
    if we are going to cut our protein (40ug) with caspase 1 or 3, how many ul of
    caspase 1...

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    Thank you for contacting Abcam.

    For ab39901, Caspase 1, we recommend using 1 unit/assay for analyzing caspase activity and this protein has a biological activity : >5000 units/mg and it is supplied lyophilized and it should be reconstituted t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"