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Product name
Anti-active Caspase 9 antibody
See all active Caspase 9 products (4) ...
Description
Rabbit polyclonal to active Caspase 9
Specificity
This antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase.
Tested applications
ICC, IP, WBmore details
Cross reactivity
Reacts with
Human
Positive control
Etoposide treated Jurkat whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 0.5% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Western blot - active Caspase 9 antibody (ab2324)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 10 - 20 µg/ml.
IP: Use a concentration of 10 - 20 µg/ml.
WB: Use a concentration of 0.5 - 1 µg/ml.
Target
Relevance
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6.
Alternative names
- APAF 3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
Database links
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
Anti-active Caspase 9 antibody images:
Western blot - active Caspase 9 antibody (ab2324)
All lanes : Anti-active Caspase 9 antibody (ab2324)
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-active Caspase 9 antibody (ab2324)
This product has been referenced in:
- Snigdha S et al. Dietary and behavioral interventions protect against age related activation of caspase cascades in the canine brain. PLoS One 6:e24652 (2011). IHC-P; Dog.Read more (PubMed: 21931796) »
- Végran F et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One 6:e29058 (2011). WB.Read more (PubMed: 22216167) »
See all 3 publications for this product
Publishing research using ab2324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"