Recombinant C4 Protease protein (ab134862)
Key features and details
- Expression system: Tobacco Vein Mottling Virus
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant C4 Protease protein -
Purity
> 95 % SDS-PAGE. -
Expression system
Tobacco Vein Mottling Virus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Tobacco vein mottling virus -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFSKALLKGVRDFNPISACVWLL ENSSDGHSERLFGIGFGPYIIANQHLFRRNNGELTIKTMHGEFKVKNSTQ LQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQPTIKDRVCMVSTNFQQKSV SSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGNILGIHSLTH TTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPE DDFMAKKTVAAIMD -
Predicted molecular weight
30 kDa including tags -
Amino acids
2002 to 2236 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab134862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
C4 Protease could be easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at N-terminal. Complete digestion 50ug of MBP-IL6 fusion protein using 1 ug TVMV C4 Protease (in 1:50 ratio) for 12 hours at 4C. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 79% PBS, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Relevance
This peptidase is present in the nuclear inclusion protein of potyviruses. TVMV C4 protease derived from Tobacco Vein Mottling Virus, recognizes a linear epitope of the general form T_V_R_F_Q / S with cleavage occurring between Q and S. The protease is used to cleave affinity tag from fusion protein. The optimal temperature for cleavage is 30 °C.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab134862 has not yet been referenced specifically in any publications.