Anti-active Caspase-3 antibody (ab49822)


  • Product nameAnti-active Caspase-3 antibody
    See all active Caspase-3 primary antibodies
  • Description
    Rabbit polyclonal to active Caspase-3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human active Caspase-3 aa 167-175 conjugated to Keyhole Limpet Haemocyanin (KLH).


    Database link: P42574

  • Positive control
    • Human epitheloid carcinoma HeLa cell line, treated with staurosporine and Human recombinant caspase-3, active.



Our Abpromise guarantee covers the use of ab49822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500.
ICC/IF 1/1000.


  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apopain antibody
    • CASP 3 antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Caspase 3 antibody
    • Caspase-3 subunit p12 antibody
    • CPP 32 antibody
    • CPP-32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • PARP cleavage protease antibody
    • Protein Yama antibody
    • SCA-1 antibody
    • SCA1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-active Caspase-3 antibody images

  • Anti-active Caspase-3 antibody (ab49822) at 1/1000 dilution

    Goat Anti-Rabbit IgG, Peroxidase conjugate
    developed using the ECL technique

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-active Caspase-3 antibody (ab49822)

ab49822 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Thymus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization No
Specification Thymus
Blocking step (agent) for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Sep 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Ferret Tissue sections (Small Intestines)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization No
Specification Small Intestines
Fixative Formaldehyde

Fabian Lean

Verified customer

Submitted Jul 19 2016

This antibody is specific to the active form of caspase 3.